I suppose I should update the people who haven't checked Smash Bros. Dojo in a while(who hasn't?)
Here is the current list of characters, all linked to their page on SBD:
Zero Suit Samus
Grand Total: 23
Currently, Super Smash Bros. Brawl boasts 23 characters, just two short of what Melee had. Obviously, Nintendo isn't going to tell us everything that is in the game, so while more characters will be announced, they will surely keep some of them a secret.
For no real reason, here is a list of the SSBM characters that have not been announced for SSBB yet:
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